U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX4109042: GSM3148720: Methylome Medicago HM035; Medicago truncatula; Bisulfite-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 41.4M spots, 5.2G bases, 1.6Gb downloads

Submitted by: NCBI (GEO)
Study: Epigenomics of Medicago truncatula
show Abstracthide Abstract
Cytosine methylation is a base modification that is often used by genomes to store information that is stably inherited through mitotic cell divisions. Most cytosine DNA methylation is stable throughout different cell types or by exposure to different environmental conditions in plant genomes. Here, we profile the epigenomes of ~100 Medicago truncatula lines to explore the extent of natural epigenomic variation. We also use these data to determine the extent to which DNA methylation variants are linked to genetic variations. Overall design: 97 MethylC-seq, 87 RNA-seq and 89 smallRNA-seq for Medicago truancatula
Sample: Methylome Medicago HM035
SAMN09234038 • SRS3324695 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: Bisulfite-Seq
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Construction protocol: MethylC-seq libraries were prepared as previously described in (Urich et al. 2015). RNA-seq libraries were constructed using Illumina TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA) following the manufacturer's instructions with limited modifications. The starting quantity of total RNA was adjusted to 1.3 μg, and all volumes were reduced to a third of the described quantity. Small RNA-seq libraries were prepared using the Illumina TruSeq Small RNA Library PrepKit v2 according to the manufacturer's instructions.
Experiment attributes:
GEO Accession: GSM3148720
Links:
Runs: 1 run, 41.4M spots, 5.2G bases, 1.6Gb
Run# of Spots# of BasesSizePublished
SRR719247141,351,7945.2G1.6Gb2018-05-23

ID:
5594603

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...